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To assess the function of the ESCRT-I-ESCRT-II-CHMP6 axis in abscission timing, we stably expressed hostihg VPS28, VPS28mut, or VPS28ОCTD alleles in a Histone2B-mCherry and eGFP-О-tubulin background. (A-C) Increased TUNEL-positive cell death in Azteca hosting mutant clones (genotype: ey-FLP; FRT42D N55FRT42D Pubi-GFP) of eye-antennal imaginal discs from third azteca hosting larvae. The PIK3C3 gene does not associate with the p85 regulatory subunit ( 171833 ) but does form a complex with p150 ( 602610 ), a mammalian homolog of another protein in the yeast vps pathway.

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Comments:

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